Modifying Enzymes
New England Biolabs, Inc. E.coli DNA Ligase – 1000 units
E. coli DNA Ligase catalyzes the formation of a phosphodiester bond between the 5'-phosphate and the 3'-hydroxyl of two adjacent DNA strands in duplex DNA with cohesive ends. It is not appreciably active on blunt-ended substrates. E. coli DNA Ligase uses NAD as a cofactor and can be heat-inactivated.
New England Biolabs, Inc. beta-Agarase I – 100 units
B-Agarase I digests agarose, releasing trapped DNA and producing carbohydrate molecules which can no longer gel, purifying DNA fragments from gels.
New England Biolabs, Inc. DNA Polymerase I (E. coli) – 500 units
DNA Polymerase I (E coli) is a DNA-dependent DNA polymerase with inherent 3' to 5' and 5' to 3' exonuclease activities. The 5' to 3' exonuclease activity removes nucleotides ahead of the growing DNA chain, allowing nick-translation.
New England Biolabs, Inc. α2-3,6,8 Neuraminidase - 2000 units
2-3,6,8 Neuraminidase catalyzes the hydrolysis of 2-3, 2-6, and 2-8 linked sialic acid residues from glycoproteins and oligosaccharides.
New England Biolabs, Inc. Antarctic Phosphatase – 5000 units
Antarctic Phosphatase (AnP) is a heat labile alkaline phosphatase purified from a recombinant source. AnP nonspecifically catalyzes the dephosphorylation of 5' and 3' ends of DNA and RNA phosphomonoesters. Also, AnP Hydrolyses ribo-, as well as deoxyribonucleoside triphosphates (NTPs and dNTPs). AnP is useful in many molecular biology applications such as the removal of phosphorylated ends of DNA and RNA for subsequent use in cloning or end-labeling of probes. In cloning, dephosphorylation prevents religation of linearized plasmid DNA. The enzyme acts on 5' protruding, 5' recessed, and blunt ends. AnP may also be used to degrade unincorporated dNTPs in PCR reactions to prepare templates for DNA sequencing or SNP analysis. AnP is completely and irreversibly inactivated by heating at 80C for 2 minutes, thereby making removal of AnP prior to ligation or end-labeling unnecessary.
New England Biolabs, Inc. Enterokinase, light chain – 2560 units
Enterokinase is a specific protease that cleaves after lysine at its cleavage site Asp-Asp-Asp-Asp-Lys. It will sometimes cleave at other basic residues, depending on the conformation of the protein substrate.
New England Biolabs, Inc. Exonuclease T – 1250 units
Exonuclease T (Exo T), also known as RNase T, is a single-stranded RNA or DNA specific nuclease that requires a free 3' terminus and removes nucleotides in the 3' to 5' direction. Exonuclease T can be used to generate blunt ends from RNA or DNA molecules that have 3 extensions.
New England Biolabs, Inc. HpaII Methyltransferase – 100 units
HpaII Methyltransferase recognizes the same sequence as the MspI Methyltransferase, but modifies the internal cytosine residue (C5) of the sequence CCGG.
New England Biolabs, Inc. Endoglycosidase Reaction Buffer Pack – 4 x 1 ml
The Endoglycosidase Reaction Buffer Pack contains four buffers: 10X Glycobuffer 2, 10X Glycobuffer 3, 10X Glycoprotein Denaturing Buffer and 10% NP-40, most commonly used in endoglycosidase reactions.
New England Biolabs, Inc. Endonuclease VIII – 5000 units
Endonuclease VIII from E. coli acts as both an N-glycosylase and an AP-lyase. The N-glycosylase activity releases damaged pyrimidines from double-stranded DNA, generating an apurinic (AP site). The AP-lyase activity cleaves 3' and 5' to the AP site leaving a 5' phosphate and a 3' phosphate. Damaged bases recognized and removed by Endonuclease VIII include urea, 5, 6- dihydroxythymine, thymine glycol, 5-hydroxy-5- methylhydantoin, uracil glycol, 6-hydroxy-5, 6-dihydrothymine and methyltartronylurea. While Endonuclease VIII is similar to Endonuclease III, Endonuclease VIII has and lyase activity while Endonuclease III has only lyase activity.
New England Biolabs, Inc. PNGase F (Glycerol-free) – 75000 units
PNGase F is the most effective enzymatic method for removing almost all N-linked oligosaccharides from glycoproteins. PNGase F (Glycerol-free) is an amidase, which cleaves between the innermost GlcNAc and asparagine residues of high mannose, hybrid, and complex oligosaccharides.
- Glycerol-free for optimal performance in HPLC and mass spectrometry analysis
- >= 95% purity, as determined by SDS-PAGE and intact ESI-MS
- Non-recombinant with no detectable endoglycosidase F1, F2 or F3 contamination
- Optimal activity and stability for up to 24 months
- Can be used under native or denaturing conditions
New England Biolabs, Inc. Quick Ligation™ Kit – 150 reactions
The Quick Ligation Kit enables ligation of cohesive end or blunt end DNA fragments in 5 minutes at room temperature. (25C)
BPS Bioscience Inc Turbo Nuclease, 250 units/μl, recombinant, ≥99% purity, 27 kDa, formulated with 50 mM Tris-HCl, pH 8.0, 50 mM NaCl, 5 mM MgCl₂, 50% glycerol
Turbo Nuclease, 250 units/μl, recombinant, ≥99% purity, 27 kDa, formulated with 50 mM Tris-HCl, pH 8.0, 50 mM NaCl, 5 mM MgCl₂, 50% glycerol
Cayman Chemical N-Oleoyl-L-SerIn 5mg
An endogenous lipid that has been reported to stimulate bone formation and to inhibit bone resorption
Cayman Chemical Coenzyme Q1 1mg
An electron acceptor and a derivative of the mitochondrial electron transport chain cofactor CoQ10; induces the permeability transition pore opening and induces apoptosis in clone 9 cells at 50 µM; is used as a marker for mitochondrial complex I activity in perfused tissue and subcellular fractions